For the convenience of users, particularly for the vast majority of experimental scientists, a publicly accessible web-server for MLCPP has been established. Moreover, to maximize users' convenience, given below a step-by-step guide on how to use it to get the desired results without the need to through the above mathematical details.

Step 1. Copy the following link thegleelab.org/MLCPP/MLCPP.html to the web browser and you will see its top page of MLCPP. Click on the ReadMe button to see a brief introduction about the server.

Step 2. Enter the query protein sequences into the input box at the center . You can also directly upload input file via the Browse button. The input sequences should be in the FASTA format. Examples of FASTA-formatted sequences can be seen by clicking on the Example button right above the input box.

Step 3. By clicking on the button submit, you will get the predicted results on the screen of your computer. The first, the second, the third and the fourth column respectively represent serial number, peptide id (i.e. user FASTA ID), Cell-penetrating or not and probability of CPP. If a given peptide is predicted as CPP, the sixth and the seventh column respectively represent uptake efficiency and probability of high uptake efficiency, otherwise “— “.

Step 4. Click on the Reset button to clear the query sequences you enter to the input box.

Step 5. Click on the Dataset button to download the datasets (training and independent) used in this paper.