For the convenience of users, particularly for the vast majority of experimental scientists, a publicly accessible web-server for THRONE has been established. Moreover, to maximize users' convenience, given below a step-by-step guide on how to use it to get the desired results without the need to through the above mathematical details. 

Step 1. Copy the following link thegleelab.org/THRONE to the web browser and you will see its top page of THRONE. Click on the ReadMe button to see a brief introduction about the server 

Step 2. Paste the query RNA sequences into the input box at the center. You can also directly upload input file via the Browse button. The input sequences should be in the FASTA format. We have also provided an example FASATA sequences. To get this sample sequence, Click Example

Step 3. By clicking on the button submit, you will get the predicted results on the screen of your computer. 

• For a given sequence, four columns are displayed. The first, the second, the third, and the fourth column represent serial number, user FASTA ID, m7G or non-m7G, and probability of m7G's.
• Notably, the probability score (ProbS) of m7G ranges from 0 to 1, where a ProbS close to 0 and 1 represent non-m7G and m7G, respectively. 

Step 4. Click on the Reset  button to clear the query sequences you enter to the input box. 

Step 5. Click on the Dataset button to download the dataset used in this paper.