For the convenience of users, particularly for the vast majority of experimental scientists, a publicly accessible web-server for STALLION has been established. Moreover, to maximize users' convenience, given below a step-by-step guide on how to use it to get the desired results without the need to through the above mathematical details. 

Step 1. Copy the following link thegleelab.org/STALLION to the web browser and you will see its top page of STALLION. Click on the ReadMe button to see a brief introduction about the server 

Step 2. Paste the query Protein sequences into the input box at the center. You can also directly upload input file via the Browse button. The input sequences should be in the FASTA format. We have also provided an example FASATA sequences. To get this sample sequence, Click Example

Step 3. We developed a Species-Specific prediction model for six different species. Therefore, the users need to select an appropriate Species type for the prediction using the drop-down menu.

Step 4. By clicking on the button submit, you will get the predicted results on the screen of your computer. 

• For the selected species-specific model, six columns are displayed. The first, the second, the third, the fourth, the fifth, and the sixth column represent serial number, user FASTA ID, K position, calculated fragment, Ace's or Non-Ace's and probability of Ace's.
• Notably, the probability score (ProbS) of Kace ranges from 0 to 1, where a ProbS close to 0 and 1 represent Kace and non-Kace, respectively. 

Step 5. Click on the Reset  button to clear the query sequences you enter to the input box. 

Step 6. Click on the Dataset button to download the datasets (training and independent) used in this paper.

STALLION server running time information for our independent dataset is given below