For the convenience of users, particularly for the vast majority of experimental scientists, a publicly accessible web-server for FRTpred has been established. Moreover, to maximize users' convenience, given below a step-by-step guide on how to use it to get the desired results without the need to through the above mathematical details. 

Step 1. Copy the following link thegleelab.org/FRTpred to the web browser and you will see its top page of FRTpred. Click on the ReadMe button to see a brief introduction about the server 

Step 2.  We developed four prediction models  that was assembled in the FRTpred framework . If the given sequence folding type is known, we recommend the user to select the appropriate model (N2SP or 2SP) prediction model. If the folding type is not known, the user have to pick combined prediction model, which will predict not only ln(kf) but also the possible folding type automatically.


Step 3. Enter the query Protein sequences into the input box at the center . You can also directly upload input file via the Browse button. The input sequences should be in the FASTA format . 


Step 4. By clicking on the button submit, you will get the predicted results on the screen of your computer. 
  • For the 2SP or N2SP model, three columns are displayed. The first, the second, and the third column respectively represent serial number, user FASTA ID, and ln(kf).
  • For the Combined model, six columns are displayed. The first, the second, the third, the fourth, and the fifth column represent serial number, user FASTA ID, ln(kf), folding class, and  probability of N2S proteins.
  • Notably, the probability score (ProbS) of N2S ranges from 0 to 1, where a ProbS close to 0 and 1 represent 2S and N2S, respectively.


Step 5. Click on the Reset  button to clear the query sequences you enter to the input box. 


Step 6. Click on the Dataset button to download the datasets (training and independent) used in this paper.